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Rat OXB (Orexin B) ELISA Kit

SKU:
AEES00490
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
Frequently bought together:

Description

Rat OXB (Orexin B) ELISA Kit

The Rat OXB (Orexin B) ELISA Kit is a highly specialized assay designed to quantitatively detect levels of Orexin B in various rat biological samples. Orexin B, also known as hypocretin 2, is a neuropeptide produced in the hypothalamus that plays a crucial role in the regulation of sleep-wake cycles, appetite, energy homeostasis, and various physiological functions. Dysregulation of Orexin B signaling has been associated with sleep disorders, metabolic imbalances, and neurological conditions. Accurate measurement of Orexin B levels using this ELISA kit is essential for studying the intricate mechanisms underlying sleep regulation, energy metabolism, and related disorders.

By quantifying Orexin B concentrations in rat samples, researchers can gain valuable insights into the role of this neuropeptide in maintaining physiological balance and its potential implications in disease pathology. The Rat OXB ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, providing precise and reproducible results. Manufactured under strict quality control guidelines, this kit delivers reliable performance with user-friendly protocols, making it an excellent choice for researchers investigating Orexin B functions and its involvement in various physiological processes.

Product Name:Rat OXB (Orexin B) ELISA Kit
Product Code:AEES00490
Assay Type:Competitive
Format:96T
Assay Time:2.0h
Reactivity:Rat
Detection Range:0.16-10 ng/mL
Sensitivity:0.10 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 50μL
Specificity:This kit recognizes OXB in samples. No significant cross-reactivity or interference between OXB and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of OXB concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OXB. During the reaction, OXB in the sample or standard competes with a fixed amount of OXB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OXB. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OXB in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level OXB were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level OXB were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)0.501.504.100.501.504.00
Standard deviation0.030.070.130.030.060.16
CV (%)6.004.673.176.004.004.00

Recovery

The recovery of OXB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)92-108100
EDTA plasma(n=8)86-9792
Cell culture media(n=8)89-10596
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

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