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Rat Osteoprotegerin (OPG) ELISA Kit (RTDL00786)

SKU:
RTDL00786
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08727
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TNFRSF11B,TNFRSF11-B,OCIF,OPG,TR1,Tumor Necrosis Factor Receptor Superfamily Member 11b,Osteoclastogenesis Inhibitory Factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649

Description

system_update_altDatasheet

Rat Osteoprotegerin (OPG) ELISA Kit

The Rat Osteoprotegerin (OPG) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of OPG levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.OPG is a key regulator of bone metabolism and plays a critical role in the pathogenesis of osteoporosis and other bone-related diseases.

By measuring OPG levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Rat Osteoprotegerin (OPG) ELISA Kit is a valuable tool for studying bone biology and exploring potential treatment strategies for skeletal disorders in rat models.

Product Name:Rat Osteoprotegerin (OPG) ELISA Kit
Product Code:RTDL00786
Size:96 Assays
Target:OPG
Synonyms:TNFRSF11B, TNFRSF11-B, OCIF, OPG, TR1, Tumor Necrosis Factor Receptor Superfamily Member 11b, Osteoclastogenesis Inhibitory Factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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