The Rat OPN (Osteopontin) ELISA Kit is meticulously tailored for the precise quantification of Osteopontin levels in a variety of rat biological samples.
Osteopontin, a multifunctional cytokine and extracellular matrix protein, is renowned for its diverse roles in cell adhesion, migration, inflammation, tissue remodeling, and immune responses. As a key modulator of physiological and pathological processes, Osteopontin is implicated in numerous conditions encompassing cancer, cardiovascular diseases, and bone metabolism. Accurate measurement of Osteopontin utilizing this ELISA kit offers researchers invaluable insights into the regulatory functions of Osteopontin in health and disease. Understanding the intricate involvement of Osteopontin in cellular signaling pathways, immune modulation, and disease progression is pivotal for discerning potential therapeutic avenues and diagnostic applications in various medical fields. The Rat OPN (Osteopontin) ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring precise and reproducible outcomes. With a focus on stringent quality control measures, this kit ensures robust performance and user-friendly operation, making it an optimal selection for cutting-edge research endeavors exploring the multifaceted roles of Osteopontin in biological systems.
Product Name:
Rat OPN (Osteopontin) ELISA Kit
SKU:
AEES00501
Target:
Rat OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OPN. You can calculate the concentration of Rat OPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
91-104
98-112
Average (%)
95
96
106
1:4
Range (%)
91-104
83-93
86-98
Average (%)
98
88
91
1:8
Range (%)
87-99
85-97
86-99
Average (%)
93
91
91
1:16
Range (%)
89-103
86-101
82-96
Average (%)
95
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
98
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.92
14.49
41.7
4.6
13.64
44.23
Standard deviation
0.26
0.7
1.53
0.3
0.74
2.28
C V (%)
5.28
4.83
3.67
6.52
5.43
5.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed.
