The Rat OPG (Osteoprotegerin) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of OPG levels in rat serum, plasma, and tissue homogenates. OPG is a key regulator of bone metabolism, inhibiting bone resorption and promoting bone formation. This kit provides accurate and reproducible results, making it an invaluable tool for research studies focused on bone health, osteoporosis, and other skeletal disorders in rats.
With its high performance and easy-to-use format, the Rat OPG CLIA Kit is ideal for a wide range of applications in the field of bone biology research.
Product Name:
Rat OPG (Osteoprotegerin) CLIA Kit
SKU:
RTES00030
Target:
Rat OPG (Osteoprotegerin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OPG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OPG. You can calculate the concentration of Rat OPG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
96-107
89-103
Average (%)
103
101
94
1:4
Range (%)
95-109
88-102
101-118
Average (%)
102
94
107
1:8
Range (%)
86-101
99-112
99-113
Average (%)
93
107
106
1:16
Range (%)
100-118
101-116
100-112
Average (%)
108
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
103
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
88-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.77
148.83
434.52
53.52
151.66
447.81
Standard deviation
4.82
15.7
39.06
6.1
11.24
46.8
C V (%)
9.31
10.55
8.99
11.4
7.41
10.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat OPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OPG in samples. No significant cross-reactivity or interference between Rat OPG and analogues was observed.
