The Rat OC BGP (Osteocalcin) CLIA Kit is a powerful tool for the precise measurement of osteocalcin levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it ideal for a variety of research purposes.Osteocalcin is a key protein in bone metabolism and is crucial for bone formation and remodeling. Abnormal levels of osteocalcin have been linked to various bone-related disorders, making it a valuable biomarker for studying these conditions and developing new treatment strategies.
Whether investigating bone health, skeletal diseases, or the effects of different treatments on bone turnover, the Rat OC BGP (Osteocalcin) CLIA Kit provides researchers with a reliable and efficient tool for their experiments. Trust in this kit to deliver reliable results and drive forward your research in the field of bone biology.
Product Name:
Rat OC/BGP (Osteocalcin) CLIA Kit
SKU:
RTES00145
Target:
Rat OC/BGP (Osteocalcin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OC/BGP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OC/BGP. You can calculate the concentration of Rat OC/BGP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
91-106
87-101
Average (%)
99
97
93
1:4
Range (%)
91-109
100-115
89-102
Average (%)
99
106
95
1:8
Range (%)
88-102
87-101
87-99
Average (%)
93
92
92
1:16
Range (%)
92-104
99-114
92-104
Average (%)
98
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
0.91
4.91
0.44
0.98
4.53
Standard deviation
0.06
0.1
0.49
0.04
0.11
0.28
C V (%)
12.5
10.99
9.98
9.09
11.22
6.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
