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Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit

SKU:
RTDL00664
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24528
Sensitivity:
27pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
Methylated-DNA--Protein-Cysteine Methyltransferase,6-O-methylguanine-DNA methyltransferase,Methylated-DNA--protein-cysteine methyltransferase
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit

The Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit is a reliable tool for detecting levels of MGMT in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results, making it perfect for a variety of research applications.MGMT is a key enzyme involved in DNA repair, specifically removing alkyl groups from the O-6 position of guanine. This process is crucial for maintaining genomic stability and preventing mutations that can lead to cancer and other diseases.

Studying MGMT levels can provide valuable insights into DNA damage repair mechanisms and potential therapeutic interventions.Overall, the Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit offers researchers a reliable and efficient way to study the role of MGMT in DNA repair processes and its implications for disease development.

Product Name:Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit
Product Code:RTDL00664
Size:96 Assays
Target:MGMT
Synonyms:Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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