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Rat Nucleobindin-2 PharmaGenie ELISA Kit (SBRS0147)

SKU:
SBRS0147
Product Type:
ELISA Kit
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Competitive
$779
Frequently bought together:

Description

Product SKU:SBRS0147
Size:96T
Application:This kit detects 420aa Nucleobindin-2 protein. Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Angiotensin II, NPY and APC.
Uniprot:Q9JI85
Gene Names:NEFA / NUCB2
Synonyms:Nucleobindin-2 (DNA-binding protein NEFA) (Prepronesfatin) [Cleaved into: Nesfatin-1]
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:147 pg/ml or 3.09 pM
Recommended Dilution:Human: 4X, Mouse: 4X, Rat: 2X
Storage/Stability:Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Standard Peptide
  4. Assay Diluent(s)
  5. Biotinylated Peptide
  6. HRP-Streptavidin
  7. TMB One-Step Substrate
  8. Stop Solution
  9. Assay Diagram
  10. Positive Control Sample
  11. Capture Antibody
  12. Technical Manual

Other materials and equipment required:

The Assay Genie Rat Nucleobindin-2 PharmaGenie ELISA Kit (SBRS0147) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 ul detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4°C.
  4. Add 100 ul standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 ul prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 ul TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul Stop Solution to each well.
  11. Read plate at 450 nm immediately.

Nucleobindin-2 has roles in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. Nucleobindin-2 binds calcium and has EF-folding domains. Nucleobindin-2 is further cleaved into a 82 amino acids peptide, Nesfatin, which plays important roles regulating food intake, energy homeostasis, and blood pressure.

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