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Rat Nucleobindin 1 (NUCB1) ELISA Kit

SKU:
RTDL00776
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63083
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NUC,CALNUC
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat Nucleobindin 1 (NUCB1) ELISA Kit

The Rat Nucleobindin 1 (NUCB1) ELISA Kit is specifically designed for the accurate and reliable detection of NUCB1 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an ideal tool for a variety of research applications.NUCB1 is a protein known for its involvement in various cellular processes, including calcium signaling, gene regulation, and stress response.

It has been associated with conditions such as obesity, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Rat Nucleobindin 1 (NUCB1) ELISA Kit offers researchers a comprehensive and efficient means of measuring NUCB1 levels in rat samples, contributing to a better understanding of its role in health and disease.

Product Name:Rat Nucleobindin 1 (NUCB1) ELISA Kit
Product Code:RTDL00776
Size:96 Assays
Target:NUCB1
Synonyms:NUC, CALNUC
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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