The Rat Nuclear Respiratory Factor 1 (NRF1) ELISA Kit is specifically designed for the precise measurement of NRF1 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.NRF1 is a key transcription factor that plays a vital role in regulating the expression of genes involved in mitochondrial biogenesis and function. It is essential for maintaining cellular energy homeostasis and oxidative stress response.
Dysregulation of NRF1 has been linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer.By using the Rat NRF1 ELISA Kit, researchers can effectively study the role of NRF1 in physiological and pathological conditions, as well as potentially identify new therapeutic targets. The reliable performance and convenience of this kit make it an indispensable tool for advancing research in the field of molecular biology and medicine.
Product Name:
Rat Nuclear Respiratory Factor 1 (NRF1) ELISA Kit
Product Code:
RTDL00760
Size:
96 Assays
Target:
NRF1
Synonyms:
ALPHA-PAL, EWG, Alpha Palindromic-Binding Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
