Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit
The Rat Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1) ELISA Kit is a comprehensive tool for the precise quantification of NR3C1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for diverse research endeavors.NR3C1, also known as the glucocorticoid receptor, is a vital nuclear receptor involved in mediating the effects of glucocorticoid hormones. Its functions encompass crucial roles in immune response regulation, metabolism, and stress responses.
Dysregulation of NR3C1 has been implicated in various disorders such as inflammation, autoimmune diseases, and psychiatric conditions, underscoring its significance as a key target for investigative studies and therapeutic interventions.By utilizing the Rat NR3C1 ELISA Kit, researchers can delve deeper into the intricate mechanisms involving NR3C1, paving the way for groundbreaking discoveries and innovative treatments in a multitude of scientific fields.
Product Name:
Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
