Rat NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen) ELISA Kit
The Rat NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen) ELISA Kit is a state-of-the-art assay designed to quantitatively measure the levels of NTXⅠ in various biological samples of rats. NTXⅠ, a specific biomarker derived from the breakdown of collagen type I in bone tissue, serves as a pivotal indicator of bone resorption and turnover. As a key component of the extracellular matrix in bone, collagen type I plays a fundamental role in maintaining bone integrity and strength. This ELISA kit empowers researchers to accurately assess NTXⅠ levels, shedding light on bone metabolism, osteoporosis, bone remodeling, and skeletal health. NTXⅠ is a reliable biomarker for monitoring bone resorption and assessing bone turnover rates, providing valuable insights into skeletal health, bone diseases, and the effectiveness of therapeutic interventions targeting bone disorders.
By utilizing the Rat NTXⅠ ELISA Kit, researchers can gain a deeper understanding of bone metabolism, osteoporotic changes, and the impact of various factors on bone health. The Rat NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen) ELISA Kit by Assay Genie ensures exceptional sensitivity and specificity, delivering reliable and reproducible results with high precision. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for investigating bone resorption, bone turnover dynamics, and skeletal health in research and clinical studies.
Product Name:
Rat NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen) ELISA Kit
SKU:
AEES00371
Target:
Rat NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NTXⅠ. You can calculate the concentration of Rat NTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
88-105
95-111
Average (%)
102
96
102
1:4
Range (%)
91-107
85-100
85-97
Average (%)
99
91
90
1:8
Range (%)
93-107
86-97
87-98
Average (%)
98
91
93
1:16
Range (%)
90-102
78-92
87-100
Average (%)
96
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.11
22.94
78.97
9.41
21.51
80.85
Standard deviation
0.6
1.31
3.29
0.61
1.29
2.9
C V (%)
6.59
5.71
4.17
6.48
6.0
3.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NTXⅠ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NTXⅠ in samples. No significant cross-reactivity or interference between Rat NTXⅠ and analogues was observed.
