Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit
The Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit is a specialized assay designed for the quantitative measurement of Neurotrophic Tyrosine Kinase Receptor Type 2 levels in various rat biological samples. NTRK2, also known as TrkB receptor, is a pivotal receptor involved in mediating the effects of neurotrophins, such as brain-derived neurotrophic factor (BDNF). It plays a crucial role in neuronal survival, differentiation, and synaptic plasticity, exerting significant influence on neurodevelopment and cognitive functions. This ELISA kit serves as a valuable tool for researchers investigating the role of NTRK2 signaling pathways in neurodevelopmental processes, neuroplasticity, and neurodegenerative diseases.
By accurately quantifying NTRK2 levels in rat samples, researchers can gain insights into the intricate mechanisms underlying neuronal growth, function, and survival, offering potential avenues for therapeutic interventions targeting neurotrophin signaling. The Rat NTRK2 ELISA Kit from Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit guarantees reliable performance and ease of use, making it a prime choice for researchers seeking to unravel the complexities of neurotrophic signaling in physiological and pathological conditions.
Product Name:
Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit
SKU:
AEES00494
Target:
Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NTRK2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NTRK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NTRK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NTRK2. You can calculate the concentration of Rat NTRK2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
97-109
96-111
Average (%)
92
103
103
1:4
Range (%)
91-102
83-99
82-92
Average (%)
96
90
87
1:8
Range (%)
89-104
81-92
86-101
Average (%)
96
87
93
1:16
Range (%)
92-105
85-98
89-103
Average (%)
99
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
285.15
934.94
2863.31
267.87
1011.54
2876.61
Standard deviation
16.99
47.4
131.14
16.88
56.04
89.46
C V (%)
5.96
5.07
4.58
6.3
5.54
3.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NTRK2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NTRK2 in samples. No significant cross-reactivity or interference between Rat NTRK2 and analogues was observed.
