The Rat NT-3 (Neurotrophin-3) CLIA Kit is a reliable and accurate tool for quantifying levels of NT-3 in rat serum, plasma, and tissue culture supernatants. With high sensitivity and specificity, this kit provides precise and reproducible results, making it an ideal choice for a variety of research applications.NT-3 is a vital neurotrophic factor that plays a crucial role in the growth, survival, and differentiation of neurons. It is involved in the development and maintenance of the nervous system and has implications in conditions such as neurodegenerative diseases and nerve injury.
Measurement of NT-3 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Get accurate and reliable results with the Rat NT-3 CLIA Kit from Assay Genie, a trusted provider of high-quality research tools.
Product Name:
Rat NT-3 (Neurotrophin 3) CLIA Kit
SKU:
RTES00404
Target:
Rat NT-3 (Neurotrophin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NT-3. You can calculate the concentration of Rat NT-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
97-115
96-111
Average (%)
93
105
103
1:4
Range (%)
104-120
88-102
87-102
Average (%)
110
94
93
1:8
Range (%)
85-97
91-106
85-97
Average (%)
90
97
92
1:16
Range (%)
97-112
87-103
94-106
Average (%)
105
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
99-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.28
226.01
732.96
96.16
227.06
693.8
Standard deviation
9.74
25.29
62.08
11.68
25.88
43.5
C V (%)
10.12
11.19
8.47
12.15
11.4
6.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NT-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-3 in samples. No significant cross-reactivity or interference between Rat NT-3 and analogues was observed.
