The Rat NRP1 (Neuropilin 1) ELISA Kit is a sophisticated diagnostic tool meticulously crafted for the precise quantification of Neuropilin 1 protein levels in diverse rat biological samples. Neuropilin 1 is a fundamental cell surface receptor involved in various cellular functions such as axon guidance, angiogenesis, and immune response modulation.
Its intricate role as a co-receptor for multiple signaling pathways underscores its significance in various physiological and pathological processes. This ELISA kit empowers researchers with the capability to quantitatively measure NRP1 levels, providing valuable insights into its expression patterns, regulatory mechanisms, and functional implications in rat models. Accurate determination of Neuropilin 1 is essential for unraveling its diverse functions, from nervous system development to cancer progression, making it an indispensable tool for dissecting complex cellular interactions. Crafted with precision and adherence to rigorous quality standards, the Rat NRP1 ELISA Kit from Assay Genie ensures unparalleled sensitivity and specificity, guaranteeing dependable and reproducible outcomes. With its user-friendly design and high-performance characteristics, this kit is a preferred choice for researchers aiming to elucidate the intricate role of Neuropilin 1 in biological systems and disease pathogenesis.
Product Name:
Rat NRP1 (Neuropilin 1) ELISA Kit
SKU:
AEES00551
Target:
Rat NRP1 (Neuropilin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NRP1. You can calculate the concentration of Rat NRP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
87-97
86-101
Average (%)
99
92
93
1:4
Range (%)
87-102
88-99
89-103
Average (%)
94
93
95
1:8
Range (%)
90-101
87-99
89-103
Average (%)
95
94
94
1:16
Range (%)
89-104
88-100
91-104
Average (%)
95
93
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
93-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.03
92.13
454.57
44.28
90.45
452.04
Standard deviation
2.66
4.68
18.64
2.81
4.82
20.93
C V (%)
5.54
5.08
4.1
6.35
5.33
4.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NRP1 in samples. No significant cross-reactivity or interference between Rat NRP1 and analogues was observed.
