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Rat NPY (Neuropeptide Y) ELISA Kit

SKU:
AEES00493
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Rat NPY (Neuropeptide Y) ELISA Kit

The Rat NPY (Neuropeptide Y) ELISA Kit is a specialized assay designed for the precise and quantitative detection of Neuropeptide Y levels in various rat biological samples. Neuropeptide Y is a key neurotransmitter and peptide hormone that plays crucial roles in modulating diverse physiological processes, including appetite regulation, stress response, cardiovascular function, and cognition. Dysregulation of Neuropeptide Y has been implicated in various conditions such as obesity, anxiety, and cardiovascular disorders, making accurate measurement of NPY levels essential for elucidating its physiological functions and pathological implications. This ELISA kit provides researchers with a powerful tool to investigate the role of Neuropeptide Y in the central nervous system, metabolic regulation, and stress responses.

By quantifying NPY levels in rat samples, researchers can enhance their understanding of the neuroendocrine system and its impact on overall health and disease states. The Rat NPY ELISA Kit from Assay Genie is characterized by exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for research studies exploring the complex functions of Neuropeptide Y in biological systems.

Product Name:Rat NPY (Neuropeptide Y) ELISA Kit
Product Code:AEES00493
Assay Type:Competitive
Format:96T
Assay Time:2.0h
Reactivity:Rat
Detection Range:31.25-2000 pg/mL
Sensitivity:18.75 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 50μL
Specificity:This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of NPY concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NPY. During the reaction, NPY in the sample or standard competes with a fixed amount of NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NPY. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPY in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

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