The Rat Notch 2 (Notch Homolog 2) ELISA Kit is a cutting-edge immunoassay designed to accurately quantify the levels of the Notch 2 protein in various biological samples obtained from rat models. Notch 2 is a critical signaling protein involved in cellular processes such as cell fate determination, differentiation, and development, playing a significant role in various physiological functions and disease pathways. This ELISA kit offers researchers a precise tool to measure and analyze the expression levels of Notch 2, providing valuable insights into its functions, molecular mechanisms, and regulatory roles in cellular activities.
Accurate quantification of Notch 2 is essential for elucidating its impact on cell signaling pathways, development, and pathological conditions. Manufactured with high-quality standards and rigorous quality control measures, the Rat Notch 2 ELISA Kit ensures exceptional sensitivity and specificity, enabling robust and reproducible results. Its user-friendly design and reliable performance make it an ideal choice for research studies investigating the role of Notch 2 in cellular processes and disease pathogenesis in rat models.
Product Name:
Rat Notch 2 (Notch Homolog 2) ELISA Kit
SKU:
AEES00550
Target:
Rat Notch 2 (Notch Homolog 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Notch 2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Notch 2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Notch 2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Notch 2. You can calculate the concentration of Rat Notch 2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
92-106
86-100
Average (%)
92
100
92
1:4
Range (%)
84-99
94-109
85-100
Average (%)
90
101
92
1:8
Range (%)
88-103
94-109
82-94
Average (%)
95
100
87
1:16
Range (%)
87-99
89-101
88-102
Average (%)
93
96
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
261.97
457.87
2406.38
247.79
490.22
2321.95
Standard deviation
14.41
21.2
121.52
15.39
25.39
99.15
C V (%)
5.5
4.63
5.05
6.21
5.18
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Notch 2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Notch 2 in samples. No significant cross-reactivity or interference between Rat Notch 2 and analogues was observed.
