Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
The Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit is a specialized assay designed to quantitatively detect the levels of endothelial nitric oxide synthase (eNOS), also known as NOS3, in various rat biological samples. Endothelial nitric oxide synthase is a critical enzyme that plays a pivotal role in endothelial function and vascular health by catalyzing the production of nitric oxide, a key signaling molecule involved in vascular tone regulation, blood pressure control, and cardiovascular homeostasis. The accurate quantification of eNOS levels using this ELISA kit can provide valuable insights into endothelial function, vascular integrity, and the pathophysiology of cardiovascular diseases.
eNOS dysfunction or dysregulation is associated with various cardiovascular disorders, such as hypertension, atherosclerosis, and coronary artery disease, making it an important target for research and therapeutic interventions. The Rat NOS3/eNOS ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and is user-friendly, making it an excellent choice for researchers investigating endothelial function, vascular biology, and cardiovascular health.
Product Name:
Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
SKU:
AEES00393
Target:
Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS3/eNOS. You can calculate the concentration of Rat NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
91-106
95-108
Average (%)
102
98
102
1:4
Range (%)
96-107
84-95
97-112
Average (%)
101
90
103
1:8
Range (%)
96-108
85-98
97-115
Average (%)
103
92
105
1:16
Range (%)
96-110
88-102
97-114
Average (%)
103
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
95-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.4
111.2
492.0
53.0
121.1
496.0
Standard deviation
3
6.7
25.1
2.7
5.2
17.9
C V (%)
6.2
6.03
5.1
5.09
4.29
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NOS3/eNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed.
