Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
The Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is a cutting-edge tool designed to quantitatively measure the levels of Nitric Oxide Synthase 2, also known as Inducible Nitric Oxide Synthase (iNOS), in various biological samples.
iNOS is a significant enzyme that plays a crucial role in the production of nitric oxide, a key signaling molecule involved in a wide range of physiological and pathophysiological processes. Accurate measurement of iNOS levels is essential for understanding its involvement in immune responses, inflammation, and diseases where nitric oxide signaling is implicated. The Rat NOS2/iNOS ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this ELISA kit provides reliable performance and user-friendly protocols, making it an excellent choice for research focusing on nitric oxide signaling pathways and related mechanisms.
Product Name:
Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
SKU:
AEES00441
Target:
Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
94-108
96-114
Average (%)
94
101
104
1:4
Range (%)
94-110
84-98
96-111
Average (%)
100
90
104
1:8
Range (%)
100-116
88-98
94-108
Average (%)
108
93
101
1:16
Range (%)
98-115
83-97
94-105
Average (%)
105
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.7
8.1
1.1
2.7
7.7
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
3.7
4.94
9.09
3.7
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.
