SLC6A2,NAT1,NET,NET1,Sodium-dependent noradrenaline transporter,Noradrenaline Transporter,Solute Carrier Family 6 member 2,Neurotransmitter transporter,Noradrenalin
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Rat Norepinephrine Transporter (NET) ELISA Kit is specifically designed for the accurate and sensitive detection of norepinephrine transporter levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The norepinephrine transporter plays a crucial role in the reuptake of norepinephrine, a neurotransmitter involved in the regulation of mood, stress response, and heart function. Dysregulation of norepinephrine transporter levels has been associated with various neurological disorders, such as depression, anxiety, and ADHD, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
With its advanced technology and reliable performance, the Rat Norepinephrine Transporter (NET) ELISA Kit is an invaluable tool for researchers looking to gain insights into the molecular mechanisms underlying norepinephrine transporter function and its implications in health and disease.
Product Name:
Rat Norepinephrine Transporter (NET) ELISA Kit
Product Code:
RTDL00723
Size:
96 Assays
Target:
NET
Synonyms:
SLC6A2, NAT1, NET, NET1, Sodium-dependent noradrenaline transporter, Noradrenaline Transporter, Solute Carrier Family 6 member 2, Neurotransmitter transporter, Noradrenalin
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
