null

Rat Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit

SKU:
RTDL00743
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5I0D6
Sensitivity:
0.031ng/ml
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
BM247 homolog,Nitric oxide synthase traffic inducer,eNOS-trafficking inducer
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit

The Rat nitric oxide synthase trafficker (Nostrin) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of Nostrin levels in rat serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.Nostrin is a key protein involved in the trafficking of nitric oxide synthase, crucial for the regulation of nitric oxide production in the body.

Dysregulation of Nostrin has been implicated in various diseases, including cardiovascular disorders, inflammatory conditions, and neurological disorders, making it a valuable biomarker for studying these diseases and developing potential treatments.Overall, the Rat Nostrin ELISA Kit provides researchers with a powerful tool for investigating the role of Nostrin in disease pathogenesis and exploring its potential as a therapeutic target.

Product Name:Rat Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit
Product Code:RTDL00743
Size:96 Assays
Target:NOSTRIN
Synonyms:BM247 homolog, Nitric oxide synthase traffic inducer, eNOS-trafficking inducer
Detection Method:Sandwich
Range:0.156-10ng/ml
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products