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Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

SKU:
RTDL00741
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06518
Sensitivity:
0.59ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
NOS2A,INOS,HEP-NOS,I-NOS,Hepatocytes Oxide Synthase,Peptidyl-cysteine S-nitrosylase NOS2
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

The Rat Nitric Oxide Synthase 2 (inducible NOS2) ELISA Kit is specifically designed for the accurate detection of inducible NOS2 levels in rat serum, plasma, and cell culture supernatants. This kit delivers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Nitric oxide synthase 2 is an important enzyme involved in the production of nitric oxide, a key signaling molecule in the body that plays a crucial role in various physiological processes.

Dysregulation of NOS2 has been linked to a range of diseases, including inflammatory disorders, neurodegenerative diseases, and cardiovascular conditions. Therefore, the Rat NOS2 ELISA Kit serves as a valuable tool for studying these conditions and exploring potential therapeutic strategies.

Product Name:Rat Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
Product Code:RTDL00741
Size:96 Assays
Target:NOS2
Synonyms:NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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