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Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit

SKU:
RTDL00744
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WV87
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MOX1,GP91-2,NOH1,NADPH Oxidase 1,Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating,NADH/NADPH mitogenic oxidase subunit P65-MOX
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit

The Rat NOX1 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of NOX1 in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.NOX1 is a key enzyme involved in the production of reactive oxygen species, playing a critical role in oxidative stress and various pathological conditions including inflammation, cardiovascular diseases, and cancer.

By measuring NOX1 levels, researchers can gain valuable insights into the role of oxidative stress in disease development and progression, as well as explore potential therapeutic targets.Overall, the Rat NOX1 ELISA Kit provides researchers with a valuable tool for studying the impact of NOX1 on various disease processes and developing novel approaches for treatment and prevention.

Product Name:Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit
Product Code:RTDL00744
Size:96 Assays
Target:NOX1
Synonyms:MOX1, GP91-2, NOH1, NADPH Oxidase 1, Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating, NADH/NADPH mitogenic oxidase subunit P65-MOX
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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