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Rat Neurotrophin 4 (NT4) ELISA Kit

SKU:
RTDL00771
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P34131
Sensitivity:
15.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
NT5,NTF4,NT-4/5,Neurotrophic Factor 4,Neurotrophin-5
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Neurotrophin 4 (NT4) ELISA Kit

The Rat Neurotrophin-4 (NT-4) ELISA Kit is a valuable tool for researchers looking to measure levels of NT-4 in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Neurotrophin-4 is a key protein involved in the development and function of the nervous system, playing a crucial role in promoting neuronal survival, differentiation, and connectivity.

Dysregulation of NT-4 has been linked to various neurological disorders, making it an important biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Rat Neurotrophin-4 (NT-4) ELISA Kit provides researchers with a reliable and effective tool for studying the role of NT-4 in neurobiology and its potential implications for disease research and treatment.

Product Name:Rat Neurotrophin 4 (NT4) ELISA Kit
Product Code:RTDL00771
Size:96 Assays
Target:NT4
Synonyms:NT5, NTF4, NT-4/5, Neurotrophic Factor 4, Neurotrophin-5
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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