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Rat Neuropathy Target Esterase (NTE) ELISA Kit

SKU:
RTDL00773
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
M0R715
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PNPLA6,Sws,Patatin-Like Phospholipase Domain Containing 6
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat Neuropathy Target Esterase (NTE) ELISA Kit

The Rat Neuropathy Target Esterase (NTE) ELISA Kit is specifically designed for the accurate measurement of NTE levels in rat serum, plasma, and tissue samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for researchers studying neuropathy and related conditions.NTE is an important enzyme involved in nerve function and has been implicated in neurological disorders such as neuropathy. By accurately measuring NTE levels, researchers can gain valuable insights into the mechanisms underlying neuropathy and potentially develop new therapeutic strategies for treatment.

With its ease of use and robust performance, the Rat NTE ELISA Kit is an essential tool for researchers investigating neuropathy and seeking to understand the role of NTE in this complex condition. Trust in this kit to deliver accurate and reproducible results for your research needs.

Product Name:Rat Neuropathy Target Esterase (NTE) ELISA Kit
Product Code:RTDL00773
Size:96 Assays
Target:NTE
Synonyms:PNPLA6, Sws, Patatin-Like Phospholipase Domain Containing 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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