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Rat Neuron Derived Orphan Receptor 1 (NOR1) ELISA Kit (RTDL00739)

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SKU:
RTDL00739
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51179
Sensitivity:
0.122ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NR4A3,NR4-A3,CHN,TEC,CSMF,MINOR,NOR1,Nuclear Receptor Subfamily 4,Group A,Member 3,Mitogen-induced nuclear orphan receptor,Nuclear hormone receptor NOR-1
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899

Description

system_update_altDatasheet

Rat Neuron Derived Orphan Receptor 1 (NOR1) ELISA Kit

The Rat Neuron-Derived Orphan Receptor 1 (NOR1) ELISA Kit is a specialized tool designed for the precise measurement of NOR1 levels in rat neuronal cells and tissues. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.NOR1 is a key nuclear receptor that regulates gene expression in neurons, playing a crucial role in neuronal development, function, and plasticity.

Dysregulation of NOR1 has been implicated in various neurologic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Rat Neuron-Derived Orphan Receptor 1 (NOR1) ELISA Kit, researchers can further their understanding of NOR1's role in neuronal biology and disease pathology, paving the way for new insights and advancements in neuroscience research.

Product Name:Rat Neuron Derived Orphan Receptor 1 (NOR1) ELISA Kit
Product Code:RTDL00739
Size:96 Assays
Target:NOR1
Synonyms:NR4A3, NR4-A3, CHN, TEC, CSMF, MINOR, NOR1, Nuclear Receptor Subfamily 4,Group A,Member 3, Mitogen-induced nuclear orphan receptor, Nuclear hormone receptor NOR-1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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