Rat Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit
The Rat Neurofilament Heavy Polypeptide (NEFH) ELISA Kit is specifically designed for the precise quantification of NEFH levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it well-suited for a variety of neuroscience research applications.NEFH is a critical component of neurofilaments, structural proteins essential for maintaining the shape and size of neurons. Dysregulation of NEFH has been implicated in various neurological disorders such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS).
Therefore, measuring NEFH levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic interventions.Overall, the Rat NEFH ELISA Kit is a valuable tool for researchers studying the role of neurofilaments in neurodegenerative diseases and exploring potential treatment strategies.
Product Name:
Rat Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit
Product Code:
RTDL00721
Size:
96 Assays
Target:
NEFH
Synonyms:
NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
