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Rat Neuregulin 1 (NRG1) ELISA Kit

SKU:
RTDL00761
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43322
Sensitivity:
0.225ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
ARIA,GGF,HGL,HRG,HRGA,NDF,SMDF,Acetylcholine receptor-inducing activity,Glial growth factor,Heregulin,Neu differentiation factor,Sensory and motor neuron-derived factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Neuregulin 1 (NRG1) ELISA Kit

The Rat Neuregulin-1 (NRG1) ELISA Kit is specially designed for precise and accurate detection of neuregulin-1 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results for a variety of research applications.Neuregulin-1 is a critical protein that plays a key role in regulating cell growth, differentiation, and survival in the nervous system. It is involved in various physiological and pathological processes, including development, synaptic function, and disease progression.

By studying neuregulin-1 levels, researchers can gain valuable insights into neurological disorders, such as schizophrenia and Alzheimer's disease, as well as potential therapeutic interventions.Overall, the Rat Neuregulin-1 (NRG1) ELISA Kit is an essential tool for researchers looking to investigate the role of neuregulin-1 in health and disease, offering reliable and accurate results for experimental studies.

Product Name:Rat Neuregulin 1 (NRG1) ELISA Kit
Product Code:RTDL00761
Size:96 Assays
Target:NRG1
Synonyms:ARIA, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor-inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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