Rat NEFL (Neurofilament, Light Polypeptide) ELISA Kit
The Rat NEFL (Neurofilament Light Polypeptide) ELISA Kit is specifically designed for the quantitative measurement of NEFL levels in rat serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.NEFL is a key component of the neurofilament protein complex found in neurons, essential for maintaining neuronal structure and function. Abnormal levels of NEFL have been linked to neurodegenerative diseases such as Alzheimer's and multiple sclerosis, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic strategies.
Overall, the Rat NEFL ELISA Kit offers a valuable tool for researchers investigating neurodegenerative disorders and seeking to understand the role of NEFL in disease progression. Its precision and versatility make it an indispensable resource for advancing scientific knowledge in the field.
Product Name:
Rat NEFL (Neurofilament, Light Polypeptide) ELISA Kit
SKU:
RTES01149
Target:
Rat NEFL (Neurofilament, Light Polypeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NEFL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NEFL. You can calculate the concentration of Rat NEFL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
84-99
89-100
Average (%)
98
90
95
1:4
Range (%)
86-100
82-94
85-96
Average (%)
93
86
90
1:8
Range (%)
86-100
87-101
86-101
Average (%)
93
92
92
1:16
Range (%)
90-106
86-97
89-102
Average (%)
97
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.4
114.37
334.38
60.53
105.61
345.32
Standard deviation
3.86
6.23
12.2
3.32
4.65
11.43
C V (%)
6.97
5.45
3.65
5.48
4.4
3.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NEFL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NEFL in samples. No significant cross-reactivity or interference between Rat NEFL and analogues was observed.
