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Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit

SKU:
RTDL00749
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41740
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GUCY2B,NPRC,ANPRC,Guanylate Cyclase C,Atrionatriuretic Peptide Receptor C,Atrial natriuretic peptide clearance receptor
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit

The Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit is specifically designed for the detection of NPR3 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.NPR3 is a receptor protein that plays a key role in the regulation of natriuretic peptides, which are essential in maintaining fluid and electrolyte balance in the body.

Dysregulation of NPR3 has been associated with cardiovascular diseases, hypertension, and other disorders related to fluid homeostasis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its high-quality components and user-friendly protocol, the Rat NPR3 ELISA Kit is a reliable tool for researchers looking to explore the role of NPR3 in health and disease.

Product Name:Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit
Product Code:RTDL00749
Size:96 Assays
Target:NPR3
Synonyms:GUCY2B, NPRC, ANPRC, Guanylate Cyclase C, Atrionatriuretic Peptide Receptor C, Atrial natriuretic peptide clearance receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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