Rat NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
The Rat NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit is a specialized assay designed for the quantitative detection of NAGase levels in various rat biological samples. NAGase is an enzyme primarily found in lysosomes and extracellular fluids, playing a crucial role in the degradation of peptides and glycoproteins containing N-acetylglucosamine moieties. Abnormal levels of NAGase have been associated with certain pathological conditions, making it a valuable biomarker for monitoring cellular damage and disease progression. Accurate measurement of NAGase using this ELISA kit is vital for researchers investigating renal and hepatic disorders, lysosomal storage diseases, and other conditions where NAGase activity is implicated.
By quantifying NAGase concentrations in rat samples, this assay enables detailed investigations into the enzyme's involvement in various physiological and pathological processes. The Rat NAGase ELISA Kit from Assay Genie provides exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for researchers studying NAGase biology and its clinical implications.
Product Name:
Rat NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
SKU:
AEES00491
Target:
Rat NAGase (N-Acetyl Beta-D-Glucosaminidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NAGase. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NAGase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NAGase. You can calculate the concentration of Rat NAGase in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
87-100
85-100
Average (%)
91
92
92
1:4
Range (%)
92-104
81-91
88-100
Average (%)
99
86
93
1:8
Range (%)
90-105
85-100
84-97
Average (%)
97
92
90
1:16
Range (%)
94-108
86-99
85-97
Average (%)
100
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.8
15.43
40.18
4.71
14.04
39.06
Standard deviation
0.3
0.82
1.99
0.28
0.57
1.2
C V (%)
6.25
5.31
4.95
5.94
4.06
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NAGase concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NAGase in samples. No significant cross-reactivity or interference between Rat NAGase and analogues was observed.
