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Rat N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit

SKU:
RTDL00720
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8VBU2
Sensitivity:
0.141ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SYLD,N-myc downstream-regulated gene 2 protein
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit

The Rat N-Myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit is specifically designed for the quantitative detection of NDRG2 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NDRG2 is an important regulator of cell proliferation, differentiation, and tumor suppression. It is involved in various cellular processes, including metastasis, hypoxia response, and neuronal differentiation.

The measurement of NDRG2 levels can provide valuable insights into the role of this protein in cancer, neurological disorders, and other diseases.By utilizing the Rat NDRG2 ELISA Kit, researchers can explore the role of NDRG2 in disease progression, identify potential therapeutic targets, and monitor treatment responses. With its reliable performance and easy-to-use format, this kit is an essential tool for studying the function and regulation of NDRG2 in rat models.

Product Name:Rat N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit
Product Code:RTDL00720
Size:96 Assays
Target:NDRG2
Synonyms:SYLD, N-myc downstream-regulated gene 2 protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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