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Rat Myosin Light Chain Kinase (MYLK) ELISA Kit

SKU:
RTDL00710
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZFU9
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KRP,MLCK108,MLCK210,MSTP083,MLCK,smMLCK,MYLK1,MLCK1,Telokin,Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form,Kinase-related protein
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Myosin Light Chain Kinase (MYLK) ELISA Kit

The Rat Myosin Light Chain Kinase (MYLK) ELISA Kit is a reliable and sensitive assay designed for the accurate quantification of MYLK levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it an ideal tool for researchers in various fields.Myosin Light Chain Kinase plays a crucial role in the regulation of smooth muscle contraction and is involved in processes such as cell migration and motility.

Dysregulation of MYLK has been linked to various diseases, including cardiovascular disorders and certain types of cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its high sensitivity and specificity, the Rat MYLK ELISA Kit offers researchers a reliable tool for studying the expression and activity of MYLK in experimental models, furthering our understanding of its role in health and disease.

Product Name:Rat Myosin Light Chain Kinase (MYLK) ELISA Kit
Product Code:RTDL00710
Size:96 Assays
Target:MYLK
Synonyms:KRP, MLCK108, MLCK210, MSTP083, MLCK, smMLCK, MYLK1, MLCK1, Telokin, Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form, Kinase-related protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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