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Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit

SKU:
RTDL00706
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02563
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ASD3,MYHC,MYHCA,MyHC-alpha,Cardiomyopathy,Hypertrophic 1,Myosin heavy chain, cardiac muscle alpha isoform
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit

The Rat Myosin Heavy Chain 6 (Cardiac Muscle Alpha) (MYH6) ELISA Kit is specifically designed for the accurate measurement of MYH6 levels in rat samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.MYH6 is an essential protein in cardiac muscle, playing a critical role in muscle contraction and cardiovascular function. Abnormalities in MYH6 expression have been linked to heart diseases and other cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

With the Rat MYH6 ELISA Kit, researchers can accurately quantify MYH6 levels in rat samples, providing valuable insights into cardiac muscle function and potential disease mechanisms. This kit is a valuable tool for cardiovascular research and drug development efforts.

Product Name:Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit
Product Code:RTDL00706
Size:96 Assays
Target:MYH6
Synonyms:ASD3, MYHC, MYHCA, MyHC-alpha, Cardiomyopathy,Hypertrophic 1, Myosin heavy chain, cardiac muscle alpha isoform
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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