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Rat Myb-related protein A (MYBL1) ELISA Kit

SKU:
RTDL00701
Product Type:
ELISA Kit
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
AMYB,A-myb,MYBA,Myb-like protein 1,v-myb avian myeloblastosis viral oncogene homolog-like 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Rat Myb-related protein A (MYBL1) ELISA Kit

The Rat MYB-Related Protein A (MYBL1) ELISA Kit is specifically designed for the precise and accurate detection of MYBL1 levels in rat serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring trustworthy and consistent results, making it perfect for a diverse range of research applications.MYBL1 is a key protein involved in regulating cell cycle progression and cell proliferation. Its dysregulation has been linked to various diseases, including cancer and developmental disorders, underscoring its importance as a critical biomarker for studying these conditions and developing potential therapeutic interventions.

Overall, the Rat MYBL1 ELISA Kit is a reliable tool for researchers looking to explore the role of MYBL1 in disease pathogenesis and potential treatment strategies. Visit the Assay Genie website to learn more about this innovative kit.

Product Name:Rat Myb-related protein A (MYBL1) ELISA Kit
Product Code:RTDL00701
Size:96 Assays
Target:MYBL1
Synonyms:AMYB, A-myb, MYBA, Myb-like protein 1, v-myb avian myeloblastosis viral oncogene homolog-like 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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