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Rat Multimerin 1 (MMRN1) ELISA Kit (RTDL00686)

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SKU:
RTDL00686
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D4A3E0
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ECM,EMILIN4,GPIa,MMRN,Glycoprotein Ia,Elastin microfibril interface located protein 4,Endothelial cell multimerin,Platelet glycoprotein Ia,155 kDa platelet multimerin
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Rat Multimerin 1 (MMRN1) ELISA Kit

The Rat Multimerin-1 (MMRN1) ELISA Kit is a reliable and sensitive assay designed for the accurate detection of Multimerin-1 levels in rat serum, plasma, and tissue lysates. This kit provides high specificity and reproducibility, making it ideal for various research applications.Multimerin-1 is a key protein involved in regulating blood clotting and platelet function, playing a crucial role in maintaining vascular health. Abnormal levels of Multimerin-1 have been associated with thrombotic disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.

With the Rat Multimerin-1 (MMRN1) ELISA Kit, researchers can confidently measure Multimerin-1 levels in rat samples with precision and accuracy, ultimately advancing their understanding of vascular biology and potential therapeutic interventions.

Product Name:Rat Multimerin 1 (MMRN1) ELISA Kit
Product Code:RTDL00686
Size:96 Assays
Target:MMRN1
Synonyms:ECM, EMILIN4, GPIa, MMRN, Glycoprotein Ia, Elastin microfibril interface located protein 4, Endothelial cell multimerin, Platelet glycoprotein Ia, 155 kDa platelet multimerin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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