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Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit

SKU:
RTDL00669
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8K4B1
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CXCL9,CMK,Humig,MI-G,SCYB9,Crg-10,Chemokine (C-X-C motif) ligand 9,Small-inducible cytokine B9,Gamma-interferon-induced monokine
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit

The Rat Monokine Induced by Interferon Gamma (MIG) ELISA Kit is a highly sensitive and specific assay designed to accurately measure levels of MIG in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.MIG is a key protein involved in the immune response, particularly in the regulation of T-cell activation and migration. Its levels have been associated with various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.

With its high quality components and user-friendly protocol, the Rat MIG ELISA Kit is a valuable tool for researchers looking to investigate the role of MIG in immune responses and disease pathogenesis. Get accurate and precise results with this innovative ELISA kit from Assay Genie.

Product Name:Rat Monokine Induced By Interferon Gamma (MIg) ELISA Kit
Product Code:RTDL00669
Size:96 Assays
Target:MIg
Synonyms:CXCL9, CMK, Humig, MI-G, SCYB9, Crg-10, Chemokine (C-X-C motif) ligand 9, Small-inducible cytokine B9, Gamma-interferon-induced monokine
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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