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Rat Monoamine Oxidase A (MAOA) ELISA Kit

SKU:
RTDL00648
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21396
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MAO-A,Amine oxidase [flavin-containing] A
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Rat Monoamine Oxidase A (MAOA) ELISA Kit

The Rat Monoamine Oxidase A (MAOA) ELISA Kit is specifically designed for the accurate and reliable detection of MAOA levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MAOA is an enzyme that plays a critical role in the metabolism of neurotransmitters such as serotonin, dopamine, and norepinephrine. Dysregulation of MAOA activity has been linked to various neurological and psychiatric disorders, making it a key target for research in these areas.

With this ELISA kit, researchers can easily quantify MAOA levels in rat samples, providing valuable insights into the role of this enzyme in disease development and progression. Overall, this kit is a valuable tool for studying the complex mechanisms underlying neurological and psychiatric conditions in rat models.

Product Name:Rat Monoamine Oxidase A (MAOA) ELISA Kit
Product Code:RTDL00648
Size:96 Assays
Target:MAOA
Synonyms:MAO-A, Amine oxidase [flavin-containing] A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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