The Rat Matrix Metalloproteinase 9 (MMP-9) ELISA Kit is a specialized assay designed to accurately quantify MMP-9 levels in various biological samples derived from rat models. MMP-9, also known as Gelatinase B, is a critical member of the matrix metalloproteinase family that plays a crucial role in the degradation and remodeling of extracellular matrix components.
This ELISA kit enables researchers to explore the activity and concentration of MMP-9, providing valuable insights into tissue remodeling, wound healing, angiogenesis, and inflammatory responses. The accurate measurement of MMP-9 levels using this kit is essential for understanding its involvement in various physiological and pathological processes, including cancer metastasis, tissue repair, and inflammatory disorders. Manufactured under strict quality control standards, this ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Researchers can rely on this kit to deliver robust performance, precise measurements, and ease of use, making it an excellent choice for investigating the role of MMP-9 in rat models and understanding its implications in different biological contexts.
Product Name:
Rat MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
SKU:
AEES00535
Target:
Rat MMP-9 (Matrix Metalloproteinase 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-9. You can calculate the concentration of Rat MMP-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-95
89-97
92-103
Average (%)
90
94
96
1:4
Range (%)
94-104
97-110
99-111
Average (%)
98
102
104
1:8
Range (%)
98-105
90-98
87-99
Average (%)
102
95
93
1:16
Range (%)
91-104
94-109
94-104
Average (%)
98
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-104
101
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
91-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.64
61.14
223.7
24.02
56.48
214.26
Standard deviation
1.41
2.78
9.91
1.21
2.8
11.48
C V (%)
5.96
4.55
4.43
5.04
4.96
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-9 in samples. No significant cross-reactivity or interference between Rat MMP-9 and analogues was observed.
