The Rat Matrix Metalloproteinase 8 (MMP-8) ELISA Kit is a specialized assay designed for the quantitative measurement of MMP-8 levels in various biological samples derived from rat models. MMP-8, also known as Collagenase-2, is a crucial member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components, particularly type I collagen.
This ELISA kit enables researchers to accurately quantify MMP-8 concentrations, providing valuable insights into tissue remodeling, inflammatory responses, wound healing, and extracellular matrix metabolism. By offering exceptional sensitivity and specificity, this kit facilitates reliable and reproducible measurement of MMP-8 levels, which is vital for understanding its role in physiological and pathological processes. Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust and consistent performance, ensuring precise and accurate measurement of MMP-8 levels in rat biological samples. With user-friendly protocols and high-performance features, this kit is an excellent tool for investigating the involvement of MMP-8 in various disease conditions, tissue remodeling processes, and inflammatory responses in rat models.
Product Name:
Rat MMP-8 (Matrix Metalloproteinase 8) ELISA Kit
SKU:
AEES00534
Target:
Rat MMP-8 (Matrix Metalloproteinase 8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-8. You can calculate the concentration of Rat MMP-8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-95
91-107
82-97
Average (%)
91
99
90
1:4
Range (%)
96-106
87-98
92-101
Average (%)
103
94
96
1:8
Range (%)
95-103
92-107
98-106
Average (%)
98
100
101
1:16
Range (%)
96-110
88-100
86-98
Average (%)
103
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
90-96
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.01
114.41
451.12
40.29
110.95
446.95
Standard deviation
2.54
5.61
22.1
2.19
5.31
21.9
C V (%)
5.77
4.9
4.9
5.44
4.79
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-8 in samples. No significant cross-reactivity or interference between Rat MMP-8 and analogues was observed.
