The Rat MMP-7 (Matrix Metalloproteinase 7) ELISA Kit is a specialized assay designed to accurately quantify the levels of Matrix Metalloproteinase 7 in various rat biological samples. Matrix Metalloproteinase 7 is an essential protease involved in tissue remodeling, cell adhesion modulation, and inflammatory responses. Its dysregulation has been associated with multiple disease conditions, making it a vital target for research and diagnostic investigations. This ELISA kit by Assay Genie offers outstanding sensitivity and specificity, enabling precise and reproducible results for your experiments.
Manufactured under rigorous quality control standards, the Rat MMP-7 ELISA Kit ensures robust performance and user-friendly protocols, facilitating the study of Matrix Metalloproteinase 7 dynamics in disease pathogenesis, wound healing, and tissue regeneration. Trust Assay Genie for high-quality tools to advance your understanding of Matrix Metalloproteinase 7 biology.
Product Name:
Rat MMP-7 (Matrix Metalloproteinase 7) ELISA Kit
SKU:
AEES00481
Target:
Rat MMP-7 (Matrix Metalloproteinase 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-7. You can calculate the concentration of Rat MMP-7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
85-96
90-105
Average (%)
105
91
97
1:4
Range (%)
100-118
83-94
92-108
Average (%)
108
88
98
1:8
Range (%)
96-110
82-94
97-111
Average (%)
103
87
104
1:16
Range (%)
97-110
87-98
98-112
Average (%)
102
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
3.4
0.5
1.3
3.3
Standard deviation
0.03
0.05
0.17
0.03
0.08
0.1
C V (%)
6
3.85
5.0
6.0
6.15
3.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-7 in samples. No significant cross-reactivity or interference between Rat MMP-7 and analogues was observed.
