The Rat MMP-7 (Matrix Metalloproteinase-7) CLIA Kit is a reliable and accurate tool for measuring MMP-7 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MMP-7 is an important enzyme involved in tissue remodeling and repair, with implications in various diseases such as cancer, inflammatory conditions, and cardiovascular disorders.
By detecting MMP-7 levels, researchers can gain valuable insights into disease pathology and potentially identify new therapeutic targets.Overall, the Rat MMP-7 CLIA Kit provides researchers with a valuable tool for studying MMP-7 biology and its role in disease, ultimately aiding in the development of innovative treatments and therapies.
Product Name:
Rat MMP-7 (Matrix Metalloproteinase 7) CLIA Kit
SKU:
RTES00385
Target:
Rat MMP-7 (Matrix Metalloproteinase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-7. You can calculate the concentration of Rat MMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-118
92-108
85-100
Average (%)
108
98
92
1:4
Range (%)
99-117
88-103
87-101
Average (%)
107
95
94
1:8
Range (%)
94-108
92-109
83-95
Average (%)
102
99
90
1:16
Range (%)
101-113
104-117
87-101
Average (%)
107
110
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.65
244.92
729.35
118.23
234.9
667.42
Standard deviation
11.0
22.7
70.53
10.63
21.75
49.92
C V (%)
10.22
9.27
9.67
8.99
9.26
7.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-7 in samples. No significant cross-reactivity or interference between Rat MMP-7 and analogues was observed.
