The Rat MMP-3 (Matrix Metalloproteinase 3) ELISA Kit is a powerful tool designed for the precise and quantitative measurement of Matrix Metalloproteinase 3 in various rat biological samples. Matrix Metalloproteinase 3, also known as Stromelysin-1, is a key enzyme involved in extracellular matrix degradation, tissue remodeling, and cell migration processes. Its dysregulation has been linked to various inflammatory and pathological conditions. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs.
Developed with stringent quality control standards, the Rat MMP-3 ELISA Kit by Assay Genie delivers reliable performance and user-friendly protocols, making it an excellent choice for investigating the role of Matrix Metalloproteinase 3 in disease progression, tissue repair, and other physiological processes. Trust Assay Genie's expertise to advance your research in Matrix Metalloproteinase 3 biology.
Product Name:
Rat MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
SKU:
AEES00480
Target:
Rat MMP-3 (Matrix Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-3. You can calculate the concentration of Rat MMP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
97-113
92-108
Average (%)
106
104
99
1:4
Range (%)
98-112
85-96
98-113
Average (%)
104
90
104
1:8
Range (%)
102-113
82-96
96-110
Average (%)
107
88
102
1:16
Range (%)
102-116
83-94
94-109
Average (%)
108
89
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.3
0.5
1.1
4.4
Standard deviation
0.03
0.06
0.19
0.03
0.06
0.15
C V (%)
6
5.45
4.42
6.0
5.45
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.
