Based on the information provided in the URL, we have the Rat MMP-2 (Matrix Metalloproteinase 2) CLIA Kit available from Assaygenie. This kit is specifically designed for the accurate detection of MMP-2 levels in rat samples, such as serum, plasma, and cell culture supernatants. The Rat MMP-2 CLIA Kit features high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying the role of MMP-2 in various biological processes. MMP-2 is a key enzyme involved in extracellular matrix degradation, cell migration, and tissue remodeling, making it a critical biomarker in pathways such as angiogenesis, wound healing, and tissue repair.
Researchers can utilize this kit to investigate the role of MMP-2 in disease states such as cancer, cardiovascular diseases, and inflammatory conditions, providing valuable insights into potential therapeutic targets. With its user-friendly protocol and high-performance characteristics, the Rat MMP-2 CLIA Kit is an essential tool for advancing research in the field of matrix metalloproteinases and their impact on various physiological and pathological processes.
Product Name:
Rat MMP-2 (Matrix Metalloproteinase 2) CLIA Kit
SKU:
RTES00381
Target:
Rat MMP-2 (Matrix Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-2. You can calculate the concentration of Rat MMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
94-108
85-98
Average (%)
103
101
91
1:4
Range (%)
99-114
90-103
88-101
Average (%)
106
96
93
1:8
Range (%)
83-95
84-98
95-109
Average (%)
90
90
101
1:16
Range (%)
91-104
95-112
101-114
Average (%)
96
103
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
181.89
639.91
1440.03
177.03
681.08
1443.91
Standard deviation
22.01
58.49
153.65
18.5
66.47
156.23
C V (%)
12.1
9.14
10.67
10.45
9.76
10.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-2 in samples. No significant cross-reactivity or interference between Rat MMP-2 and analogues was observed.
