Rat MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
The Rat Matrix Metalloproteinase 13 (MMP-13) ELISA Kit is innovatively designed to quantitatively detect MMP-13 levels in a variety of rat biological samples. MMP-13, a prominent member of the matrix metalloproteinase family, plays a crucial role in extracellular matrix remodeling by cleaving various matrix components. With its involvement in tissue homeostasis, repair, and inflammatory processes, MMP-13 serves as a key regulator in maintaining tissue integrity and function.
This ELISA kit provides researchers with a reliable and accurate tool for assessing MMP-13 levels, offering valuable insights into its contribution to physiological and pathological conditions, including arthritis, cancer metastasis, and tissue regeneration. Boasting exceptional sensitivity and specificity, the assay ensures robust and reproducible results, facilitating in-depth investigations into MMP-13 biology and its implications in various disease mechanisms. Manufactured under strict quality controls, this kit promises reliable performance, making it a preferred choice for research studies focused on matrix dynamics and tissue remodeling.
Product Name:
Rat MMP-13 (Matrix Metalloproteinase 13) ELISA Kit
SKU:
AEES00306
Target:
Rat MMP-13 (Matrix Metalloproteinase 13)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-13. You can calculate the concentration of Rat MMP-13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
87-103
86-97
Average (%)
102
94
92
1:4
Range (%)
88-104
84-96
83-96
Average (%)
95
89
88
1:8
Range (%)
90-102
85-98
86-101
Average (%)
95
90
93
1:16
Range (%)
90-107
83-97
86-100
Average (%)
98
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
1.71
8.02
0.9
1.7
8.38
Standard deviation
0.06
0.09
0.41
0.05
0.09
0.43
C V (%)
6.45
5.26
5.11
5.56
5.29
5.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-13 in samples. No significant cross-reactivity or interference between Rat MMP-13 and analogues was observed.
