The Rat MMP-1 (Matrix Metalloproteinase 1) ELISA Kit is a powerful tool designed for the precise and quantitative measurement of Matrix Metalloproteinase 1 levels in various rat biological samples. Matrix Metalloproteinase 1 is a key enzyme responsible for degrading components of the extracellular matrix, playing essential roles in tissue remodeling, wound healing, and various physiological processes. This ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research experiments.
Manufactured under strict quality control to deliver reliable and robust performance, the Assay Genie's MMP-1 ELISA Kit offers researchers a user-friendly and effective solution to investigate the functions and regulatory mechanisms of Matrix Metalloproteinase 1 in biological pathways and disease conditions. Trust in Assay Genie for high-quality ELISA kits that drive cutting-edge research in Matrix Metalloproteinase biology.
Product Name:
Rat MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
SKU:
AEES00478
Target:
Rat MMP-1 (Matrix Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-1. You can calculate the concentration of Rat MMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
95-107
86-98
Average (%)
97
102
93
1:4
Range (%)
98-114
84-95
98-110
Average (%)
106
89
104
1:8
Range (%)
95-109
81-93
94-108
Average (%)
100
87
102
1:16
Range (%)
99-116
81-95
95-106
Average (%)
106
87
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.0
4.5
0.5
1.0
4.6
Standard deviation
0.03
0.05
0.14
0.03
0.06
0.2
C V (%)
6
5.0
3.11
6.0
6.0
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-1 in samples. No significant cross-reactivity or interference between Rat MMP-1 and analogues was observed.
