Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit
The Rat Mixed Lineage Kinase Domain-Like Protein (MLKL) ELISA Kit is a specialized assay designed for the quantification of MLKL levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.MLKL is a key protein involved in cell death pathways, particularly in necroptosis, a form of programmed cell death. Dysregulation of MLKL has been linked to various diseases, including inflammatory disorders, neurodegenerative conditions, and cancer.
As such, measuring MLKL levels can provide valuable insights into disease progression and potential therapeutic targets.With its high-quality components and user-friendly protocol, the Rat MLKL ELISA Kit is an indispensable tool for researchers studying cell death mechanisms and exploring novel treatment strategies in a rat model system. Experience the reliability and precision of this kit in your research endeavors today.
Product Name:
Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit
Product Code:
RTDL00675
Size:
96 Assays
Target:
MLKL
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
