Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
The Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit is a specialized assay tailored for the quantitative measurement of MIP-1α levels in rat biological samples.
MIP-1α is a key chemokine involved in orchestrating inflammatory responses, particularly in recruitment and activation of immune cells, such as monocytes and lymphocytes, in various physiological and pathological conditions. Our ELISA kit offers a precise and reliable method for detecting and quantifying MIP-1α levels, enabling researchers to gain insights into the immune response modulation, inflammation processes, and disease pathogenesis. With exceptional sensitivity and specificity, the Assay Genie's IL-1 ELISA Kit ensures accurate and reproducible results, setting a gold standard for research in immunology, inflammation, and related fields. Trust in our kit to deliver robust performance and ease of use for your investigative needs.
Product Name:
Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
SKU:
AEES00472
Target:
Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MIP-1α. You can calculate the concentration of Rat MIP-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
94-105
92-105
Average (%)
101
100
97
1:4
Range (%)
98-113
88-98
92-105
Average (%)
106
93
100
1:8
Range (%)
95-108
84-96
97-112
Average (%)
101
90
104
1:16
Range (%)
98-111
83-97
91-103
Average (%)
105
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.6
298.5
924.4
97.0
296.8
958.2
Standard deviation
5.2
12.8
36.1
5.1
14.5
38.3
C V (%)
4.97
4.29
3.91
5.26
4.89
4.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MIP-1α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MIP-1α in samples. No significant cross-reactivity or interference between Rat MIP-1α and analogues was observed.
