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Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit

SKU:
AEES00486
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit

The Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit is a specialized assay designed for the quantitative analysis of MCP-3 levels in various rat biological samples. MCP-3, also known as CCL7, is a chemokine involved in immune cell recruitment, particularly in the attraction of monocytes and lymphocytes to sites of inflammation.

It regulates the migration and activation of these immune cells, contributing to the body's immune response and inflammatory processes. This ELISA kit offers researchers a reliable method to measure and quantify MCP-3 levels in rat samples. By accurately detecting MCP-3, researchers can gain valuable insights into immune cell behaviors, inflammatory responses, and pathological conditions where MCP-3 plays a role. The Rat MCP-3 ELISA Kit provides high sensitivity and specificity, ensuring robust and reproducible results. With its stringent quality control standards, this kit is optimized for performance and ease of use, making it an excellent choice for research applications focused on immune cell recruitment and inflammation regulation in rat models.

Product Name:Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
Product Code:AEES00486
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat MCP-3 in samples. No significant cross-reactivity or interference between Rat MCP-3 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat MCP-3 concentrations in Serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-3. You can calculate the concentration of Rat MCP-3 in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MCP-3 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MCP-3 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)47.76101.42379.9750.2197.38398.22
Standard deviation2.715.7912.203.045.4112.19
CV (%)5.675.713.216.055.563.06

Recovery

The recovery of Rat MCP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)96-110101
EDTA plasma (n=8)93-106100
Cell culture media (n=8)93-109100
Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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