Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
The Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit is a specialized tool designed for the quantitative measurement of MCP-1 levels in various rat biological samples.
Monocyte Chemotactic Protein 1, also known as CCL2, plays a vital role in immune responses as a potent chemoattractant for monocytes, memory T cells, and dendritic cells. It is involved in the regulation of immune cell migration to sites of inflammation and injury, thus contributing to the immune defense and inflammatory processes in the body. This ELISA kit enables researchers to accurately detect and quantify MCP-1 levels in rat samples, providing valuable insights into the immune response, inflammation, and various disease conditions where MCP-1 is involved. With exceptional sensitivity and specificity, this assay ensures reliable and reproducible results. Manufactured to high quality standards, the Rat MCP-1 ELISA Kit offers robust performance and ease of use, making it an ideal choice for research applications focused on immune responses and inflammatory pathways in rat models.
Product Name:
Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
SKU:
AEES00485
Target:
Rat MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
92-107
94-106
Average (%)
101
100
100
1:4
Range (%)
94-109
82-94
95-107
Average (%)
101
88
101
1:8
Range (%)
95-108
81-93
95-108
Average (%)
101
86
100
1:16
Range (%)
98-118
82-95
95-111
Average (%)
107
88
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.4
0.5
1.2
4.7
Standard deviation
0.03
0.05
0.15
0.03
0.07
0.17
C V (%)
6
4.55
3.41
6.0
5.83
3.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed.
