The Rat MBP (Myelin Basic Protein) ELISA Kit is a specialized assay designed for the quantitative detection of Myelin Basic Protein levels in various rat biological samples.
Myelin Basic Protein is critically involved in the formation and maintenance of myelin, the protective sheath surrounding nerve cells that enables efficient nerve signal transmission. This protein plays a vital role in the central nervous system, contributing to the insulation and integrity of nerve fibers. By accurately measuring Myelin Basic Protein levels, researchers can gain insights into the integrity of myelin sheaths, demyelinating diseases, and neurological disorders affecting the nervous system. The Rat MBP ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results in studying myelin-related pathologies and neurodegenerative conditions in rat models. Manufactured under strict quality control standards, this kit provides reliable performance and user-friendly protocols, making it a valuable tool for research focused on neural health and disorders associated with myelin dysfunction.
Product Name:
Rat MBP (Myelin Basic Protein) ELISA Kit
SKU:
AEES00488
Target:
Rat MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MBP. You can calculate the concentration of Rat MBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
99-114
88-101
Average (%)
102
106
95
1:4
Range (%)
93-105
81-92
84-100
Average (%)
98
85
91
1:8
Range (%)
92-108
82-95
83-96
Average (%)
100
87
88
1:16
Range (%)
87-101
86-99
83-95
Average (%)
92
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.25
4.11
0.53
1.31
4.32
Standard deviation
0.03
0.07
0.14
0.03
0.07
0.2
C V (%)
6
5.6
3.41
5.66
5.34
4.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
